CD8 [C8/1779R]

CD8 [C8/1779R] is a rabbit monoclonal antibody that is intended for laboratory use in the qualitative identification of CD8a protein by immunohistochemistry (IHC) in formalin-fixed paraffin-embedded (FFPE) human tissues. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.

CD8 is a cell surface glycoprotein member of the immunoglobulin superfamily. CD8 consists of two chains, alpha and beta, which are expressed as a disulfide-linked alpha/beta heterodimer or as an alpha/alpha homodimer on a subset of T-cells, thymocytes and NK cells (1). The majority of CD8+ T cells express CD8 as alpha/beta heterodimer. CD8 functions as a co-receptor in concert with T cell receptor for binding the MHC class I/peptide complex (1). CD8 has been shown to be an important marker to analyze T-cell mediated inflammatory dermatoses, is useful for analysis of mycosis fungoides and other T cell lymphomas, and plays a critical role in anti-tumor responses and tumor microenvironment (2-4).

Specifications

1. Williamson SL, et al . New monoclonal antibodies to the T cell antigens CD4 and CD8. Production and characterization in formalin-fixed paraffinembedded tissue. Am J Pathol. 1998 Jun;152(6):1421-6.
2. Deguchi M, et al . Proliferative activity of CD8(+) T cells as an important clue to analyze T cell-mediated inflammatory dermatoses. Arch Dermatol Res. 2001 Sep;293 (9):442-7.
3. Izban KF, et al . Immunohistochemical analysis of mycosis fungoides on paraffin-embedded tissue sections. Mod Pathol. 1998 Oct;11(10):978-82.
4. Taube, JM, et al . Implications of the tumor immune microenvironment for staging and therapeutics. Mod Pathol. 2018;31:214-34.
5. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
6. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.